- In "Hero in Residence", two elder guardians appear in the Sea Temple to determine if Jesse is worthy of getting the Structure Block. They are killed by both Petra and Jesse afterwards.
- The Elder Guardians are often incorrectly referred to as "guardians" by the main characters: Jesse, Petra, and Jack, specifically.
- In Minecraft Story Mode, Elder Guardians are shown to be helpless out of water. This is contrasting to regular Minecraft, where they can endlessly hop around and fire laser beams just fine on land.